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Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, <t>Sha31</t> (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs
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Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, <t>Sha31</t> (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs
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Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, <t>Sha31</t> (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs
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Average 90 stars, based on 1 article reviews
anti-prp monoclonal antibodies sha31 - by Bioz Stars, 2026-08
90/100 stars
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Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, <t>Sha31</t> (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs
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Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, Sha31 (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs

Journal: Acta Neuropathologica

Article Title: Transmission of cervid prions to humanized mice demonstrates the zoonotic potential of CWD

doi: 10.1007/s00401-022-02482-9

Figure Lengend Snippet: Biochemical characteristics of protease-resistant hCWD-prion protein of Wisc-1-inoculated tg650 mice. Western blot analysis of brain homogenates of tg650 mice inoculated with Wisc-1 isolate and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, Sha31 (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3013, as well as a positive control (sCJD, MM1 subtype) were also included in the Western blot. Higher exposure for mAb 9A2 is also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs

Article Snippet: PVDF membranes were blocked for 1 h in PBS-Tween (0.1%) containing skim milk powder (5%) and probed using anti-PrP monoclonal antibodies Sha31 (aa 145–152; 1:15,000; Cayman), 12F10 (aa 145–155; 1:200; Cayman), and 9A2 (aa 102–104) and 12B2 (aa 93–97; 1:1000; Wageningen Bioveterinary Research) overnight at 4 °C.

Techniques: Western Blot, Negative Control, Positive Control

Transmission of CWD-tg650 brain/spinal cord homogenates from mouse #327 to tg650 mice. Western blot analysis of brain homogenates from tg650 mouse (#3063; 2nd passage) inoculated with brain/spinal cord homogenates from tg650-Wisc-1 #327 (1st passage) and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, Sha31 (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3011, as well as a positive control (sCJD) were included in the Western blot. Higher exposures for mAbs, 12F10 and 9A2, are also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs

Journal: Acta Neuropathologica

Article Title: Transmission of cervid prions to humanized mice demonstrates the zoonotic potential of CWD

doi: 10.1007/s00401-022-02482-9

Figure Lengend Snippet: Transmission of CWD-tg650 brain/spinal cord homogenates from mouse #327 to tg650 mice. Western blot analysis of brain homogenates from tg650 mouse (#3063; 2nd passage) inoculated with brain/spinal cord homogenates from tg650-Wisc-1 #327 (1st passage) and digested with 200 µg/mL of PK using anti-PrP mAbs, from top to bottom, Sha31 (aa 145–152), 12F10 (aa 145–155), 9A2 (aa 102–104), and 12B2 (aa 93–97). A negative control, tg650 #3011, as well as a positive control (sCJD) were included in the Western blot. Higher exposures for mAbs, 12F10 and 9A2, are also shown. The bands seen at 25 kDa are nonspecific bands that react with certain Abs

Article Snippet: PVDF membranes were blocked for 1 h in PBS-Tween (0.1%) containing skim milk powder (5%) and probed using anti-PrP monoclonal antibodies Sha31 (aa 145–152; 1:15,000; Cayman), 12F10 (aa 145–155; 1:200; Cayman), and 9A2 (aa 102–104) and 12B2 (aa 93–97; 1:1000; Wageningen Bioveterinary Research) overnight at 4 °C.

Techniques: Transmission Assay, Western Blot, Negative Control, Positive Control